The assay time is considerably reduced from about an hour to few minutes by the newly reported RTF-EXPAR method which uses reverse transcriptase-free (RTF) approach for conversion of RNA ເຂົ້າໄປໃນ DNA followed by EXPAR (Exponential Amplification Reaction) for amplification at single temperature.
Controlling the rate of Covid-19 spread requires an accurate and a faster virus testing strategy. RT-PCR (reverse transcriptase polymerase chain reaction), the most accurate testing method being currently employed is a two-step test that takes around 60 minutes per sample.
ນັກຄົ້ນຄວ້າຈາກມະຫາວິທະຍາໄລ Birmingham ໄດ້ລາຍງານວິທີການໃຫມ່ສໍາລັບການກວດຫາ SARS-CoV-2. ນີ້ສາມາດເຮັດໃຫ້ການທົດສອບໄວຂຶ້ນຫຼາຍ ແລະມີຄວາມອ່ອນໄຫວພຽງພໍ.
ການກວດຫາ RNA ໄວຣັສໂດຍ RT-PCR (ຕິກິຣິຍາຕ່ອງໂສ້ transcriptase polymerase ແບບປີ້ນກັບກັນ) ກ່ຽວຂ້ອງກັບການປ່ຽນ RNA ຂອງໄວຣັດໄປສູ່ DNA ເສີມ (cDNA) ຕິດຕາມດ້ວຍການຂະຫຍາຍຂອງ cDNA ໂດຍ PCR ປະລິມານ (qPCR). ຫຼັງຈາກນັ້ນ, cDNA ໄດ້ຖືກກວດພົບໂດຍໃຊ້ສີຍ້ອມ fluorescent. ອັນນີ້ໃຊ້ເວລາເຖິງໜຶ່ງຊົ່ວໂມງ.
The assay time is considerably reduced from about an hour to few minutes by the newly reported RTF-EXPAR method which uses reverse transcriptase-free (RTF) approach for conversion of RNA into DNA followed by EXPAR (Exponential Amplification Reaction) for amplification at single temperature. The amplification taking place at a single temperature is the key to speed, because it avoids lengthy heating and cooling steps of RT-PCR. Further, the section of DNA being amplified is smaller compared RT-PCR. Hence, EXPAR generates up to 108 strands of DNA product in few minutes. The duplex formation is monitored, as in RT-PCR method using fluorescent dye, SYBR Green.
ຫນ້າສົນໃຈ, ວິທີການໃຫມ່ສາມາດຖືກດັດແປງເພື່ອກວດຫາພະຍາດຕິດຕໍ່ອື່ນໆທີ່ເກີດຈາກໄວຣັສ RNA, ຕົວຢ່າງ Ebola, RSV, ແລະອື່ນໆ.
ແຫຼ່ງຂໍ້ມູນ:
Carter et al (2020). ການກວດຫາໄລຍະ 5 ນາທີຂອງ SARS-CoV-2 RNA ໂດຍໃຊ້ Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR. ພິມລ່ວງໜ້າ. ຈັດພີມມາຢູ່ທີ່ medRxiv ຈັດພີມມາໃນວັນທີ 04 ມັງກອນ 2021. DOI: https://doi.org/10.1101/2020.12.31.20248236
***
